The Loi de Beer-Lambert is a fundamental principle in spectrophotometry that relates the absorption of light to the properties of the material through which the light is traveling. This law is crucial for determining the concentration of solutions using spectrophotometric measurements. The document covers key aspects of the Beer-Lambert law, including its formula, applications in color analysis, and the creation of calibration curves for concentration determination.
• The law is expressed as A = kC, where A is absorbance, k is a constant, and C is concentration.
• It can also be written as A = ε × l × C, where ε is the molar extinction coefficient and l is the path length.
• The law is applied in analyzing solution colors, creating calibration curves, and calculating concentrations.
• Practical applications include dilution series preparation and graphical analysis of absorbance vs. concentration.
